| Storage and stability: The components of the kit, when stored at room temperature (≤ 25ºC), will remain stable until the expiry date stated on the label. Working reagent is stable for up to 15 days at room temperature (≤25ºC). Store in the dark. - Kit Contents: * Urea reagent (R1) 4 x 100 ml * Urea reagent (R2) 2 x 50 ml * Urea Standard 1 x 5 ml |
| Name | Urea |
| Description | More than 90% of urea is excreted through the kidneys in urines. Measurement of the plasma or serum urea concentration is widely regarded as a test of renal function. However, a number of non-renal factors also influence the circulating urea concentration: Urea increased level occurs when proteins catabolism is accelerated, burns, stress, myocardial infarction... Urea is decreased in acute liver destruction and is accompanied with increased ammonium level. Urea level is generally studied in conjunction with creatinine level (urea/creatinine ratio) to refine the diagnosis of post-renal or pre-renal azotemia. |
| Principle | Urease-GLDH kinetic method is a UV-test for the quantitative determination of urea in serum, plasma, or urine, typically measured at 340 nm. It works by enzymatically converting urea to ammonium ions, which then react with 2-oxoglutarate and NADH in the presence of glutamate dehydrogenase (GLDH), causing a decrease in NADH absorbance, which is proportional to the urea concentration. |
Tags: Urea, Test, Kit, Laboratory
